Sing Lee , PhD
Education
Ph.D., Chemistry, Michigan State University, 2003
Bachelor of Science, Chemistry, Hong Kong University of Science and Technology, 2000
Employment
Associate Professor, Michigan State University, East Lansing, 2024 - Present
Associate Professor, Michigan State University, East Lansing, 2024 - Present
Assistant Professor, Michigan State University, East Lansing, 2019 - Present
Assistant Professor, Michigan State University, East Lansing, 2017 - Present
Project Scientist, University of California Davis, Davis, 2014 - 2016
Post-doctoral Scholar, University of California Davis, Davis, 2010 - 2014
Publications
Relative Importance of Soluble and Microsomal Epoxide Hydrolases for the Hydrolysis of Epoxy-Fatty Acids in Human Tissues International Journal of Molecular Sciences (2021)
Movement to the Clinic of Soluble Epoxide Hydrolase Inhibitor EC5026 as an Analgesic for Neuropathic Pain and for Use as a Nonaddictive Opioid Alternative Journal of Medicinal Chemistry (2021)
Cytochrome P450 Metabolism of Polyunsaturated Fatty Acids and Neurodegeneration Nutrients (2020)
Preparation and evaluation of soluble epoxide hydrolase inhibitors with improved physical properties and potencies for treating diabetic neuropathic pain Bioorganic & Medicinal Chemistry (2020)
Inactivation of Cys 674 in SERCA2 increases BP by inducing endoplasmic reticulum stress and soluble epoxide hydrolase British Journal of Pharmacology (2020)
Asymmetric Total Synthesis of 19,20-Epoxydocosapentaenoic Acid, a Bioactive Metabolite of Docosahexaenoic Acid The Journal of Organic Chemistry (2019)
Enzymatic Synthesis of Epoxidized Metabolites of Docosahexaenoic, Eicosapentaenoic, and Arachidonic Acids Journal of Visualized Experiments (2019)
Brain oxylipin concentrations following hypercapnia/ischemia: effects of brain dissection and dissection time J Lipid Res (2019)
Drug-Target Residence Time Affects in Vivo Target Occupancy through Multiple Pathways ACS Cent Sci (2019)
Targeted Metabolomics Identifies the Cytochrome P450 Monooxygenase Eicosanoid Pathway as a Novel Therapeutic Target of Colon Tumorigenesis Cancer Res (2019)
trans, trans-2,4-decadienal, a lipid peroxidation product, induces inflammatory responses via Hsp90- or 14–3-3ζ-dependent mechanisms The Journal of Nutritional Biochemistry (2019)
Enzymatic synthesis and chemical inversion provide both enantiomers of bioactive epoxydocosapentaenoic acids J Lipid Res (2018)
Soluble epoxide hydrolase inhibition decreases reperfusion injury after focal cerebral ischemia Sci Rep (2018)
A new sensitive LC/MS/MS analysis of vitamin D metabolites using a click derivatization reagent, 2-nitrosopyridine J Lipid Res (2017)
Active-Site Flexibility and Substrate Specificity in a Bacterial Virulence Factor: Crystallographic Snapshots of an Epoxide Hydrolase Structure (2017)
Chemical synthesis and biological evaluation of omega-hydroxy polyunsaturated fatty acids Bioorg Med Chem Lett (2017)
Cyclooxygenase-derived proangiogenic metabolites of epoxyeicosatrienoic acids Proc Natl Acad Sci U S A (2017)
Cytochrome P450 monooxygenase lipid metabolites are significant second messengers in the resolution of choroidal neovascularization Proc Natl Acad Sci U S A (2017)
Endothelial Nox4-based NADPH oxidase regulates atherosclerosis via soluble epoxide hydrolase Biochim Biophys Acta Mol Basis Dis (2017)
Epoxide metabolites of arachidonate and docosahexaenoate function conversely in acute kidney injury involved in GSK3beta signaling Proc Natl Acad Sci U S A (2017)
Epoxy fatty acids mediate analgesia in murine diabetic neuropathy Eur J Pain (2017)
Inhibition of soluble epoxide hydrolase augments astrocyte release of vascular endothelial growth factor and neuronal recovery after oxygen-glucose deprivation J Neurochem (2017)
Linoleic acid participates in the response to ischemic brain injury through oxidized metabolites that regulate neurotransmission Sci Rep (2017)
Probing the orientation of inhibitor and epoxy-eicosatrienoic acid binding in the active site of soluble epoxide hydrolase Arch Biochem Biophys (2017)
Soluble Epoxide Hydrolase Pharmacological Inhibition Decreases Alveolar Bone Loss by Modulating Host Inflammatory Response, RANK-Related Signaling, Endoplasmic Reticulum Stress, and Apoptosis J Pharmacol Exp Ther (2017)
Soluble epoxide hydrolase in podocytes is a significant contributor to renal function under hyperglycemia Biochim Biophys Acta Gen Subj (2017)
Synthesis of cyclooxygenase metabolites of 8,9-epoxyeicosatrienoic acid (EET): 11- and 15-hydroxy 8,9-EETs Org Biomol Chem (2017)
omega-3 Polyunsaturated fatty acids and their cytochrome P450-derived metabolites suppress colorectal tumor development in mice J Nutr Biochem (2017)
Cytochrome P450 Oxidase 2C Inhibition Adds to omega-3 Long-Chain Polyunsaturated Fatty Acids Protection Against Retinal and Choroidal Neovascularization Arterioscler Thromb Vasc Biol (2016)
Ingestion of the epoxide hydrolase inhibitor AUDA modulates immune responses of the mosquito, Culex quinquefasciatus during blood feeding Insect Biochem Mol Biol (2016)
Molecular Mechanisms and New Treatment Paradigm for Atrial Fibrillation Circ Arrhythm Electrophysiol (2016)
Novel Omega-3 Fatty Acid Epoxygenase Metabolite Reduces Kidney Fibrosis Int J Mol Sci (2016)
Pro-atherogenic role of smooth muscle Nox4-based NADPH oxidase J Mol Cell Cardiol (2016)
Soluble Epoxide Hydrolase Inhibition and Epoxyeicosatrienoic Acid Treatment Improve Vascularization of Engineered Skin Substitutes Plast Reconstr Surg Glob Open (2016)
Soluble epoxide hydrolase inhibitor 1-trifluoromethoxyphenyl-3- (1-propionylpiperidin-4-yl) urea attenuates bleomycin-induced pulmonary fibrosis in mice Cell Tissue Res (2016)
"Turn-on" protein fluorescence: in situ formation of cyanine dyes J Am Chem Soc (2015)
Endoplasmic reticulum stress in the peripheral nervous system is a significant driver of neuropathic pain Proc Natl Acad Sci U S A (2015)
Identification of potent inhibitors of the chicken soluble epoxide hydrolase Bioorg Med Chem Lett (2015)
Method for Sorting and Pairwise Selection of Nanobodies for the Development of Highly Sensitive Sandwich Immunoassays Anal Chem (2015)
PPARdelta signaling mediates the cytotoxicity of DHA in H9c2 cells Toxicol Lett (2015)
PPAR delta signaling mediates the cytotoxicity of DHA in H9c2 cells Toxicology Letters (2015)
An omega-3 epoxide of docosahexaenoic acid lowers blood pressure in angiotensin-II-dependent hypertension J Cardiovasc Pharmacol (2014)
Effect of soluble epoxide hydrolase polymorphism on substrate and inhibitor selectivity and dimer formation J Lipid Res (2014)
Optimized inhibitors of soluble epoxide hydrolase improve in vitro target residence time and in vivo efficacy J Med Chem (2014)
Symmetric adamantyl-diureas as soluble epoxide hydrolase inhibitors Bioorg Med Chem Lett (2014)
Epoxy metabolites of docosahexaenoic acid (DHA) inhibit angiogenesis, tumor growth, and metastasis Proc Natl Acad Sci U S A (2013)
Forster resonance energy transfer competitive displacement assay for human soluble epoxide hydrolase Anal Biochem (2013)
Rational design of a colorimetric pH sensor from a soluble retinoic acid chaperone J Am Chem Soc (2013)
Unique mechanistic insights into the beneficial effects of soluble epoxide hydrolase inhibitors in the prevention of cardiac fibrosis Proc Natl Acad Sci U S A (2013)
Probing Wavelength Regulation with an Engineered Rhodopsin Mimic and a C15-Retinal Analogue Chempluschem (2012)
Tuning the electronic absorption of protein-embedded all-trans-retinal Science (2012)
Dissection of the critical binding determinants of cellular retinoic acid binding protein II by mutagenesis and fluorescence binding assay Proteins (2009)
Elucidating the exact role of engineered CRABPII residues for the formation of a retinal protonated Schiff base Proteins (2009)
Remarkable axial ligand effect on regioselectivity towards terminal alkenes in epoxidation of dienes by a robust manganese porphyrin Chemical Communications (2003)